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You are trying to establish the IgG response from a recently vaccinated individual, where it will...

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Chemistry

You are trying to establish the IgG response from a recentlyvaccinated individual, where it will be important to know how muchIgG they have both before and after the vaccination. You start withtwo samples of 8 mL of blood, one from before vaccination and oneafter. You use an affinity chromatography technique to remove justthe IgG (ε = 1.35 g-1L cm-1). You end with a 2 mL sample of each.The pre-vaccinated sample has an A280 reading of 0.85, and thepost-vaccinated sample has an A280 reading of 0.202 with a 10-folddilution (both with a 1 cm path length).

a) How many micrograms of IgG were in each of theoriginal blood samples?

b) What fold change in IgG amounts was seen aftervaccination?

Answer & Explanation Solved by verified expert
4.0 Ratings (718 Votes)
Ans BeerLamberts Law A e C l equation 1 where A Absorbance e molar absorptivity at specified wavelength l path length in cm C Molar concentration of the solute Given e 135 g1L cm1 1350 mg1L cm1 1 g 1000 mg A before vaccination 085 A After    See Answer
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