You have 200 ml of 0.1M HEPES buffer, pH 7.5. You add 2 ml of
0.4M...
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Chemistry
You have 200 ml of 0.1M HEPES buffer, pH 7.5. You add 2 ml of0.4M HCl. The pKa of HEPES is 7.5.
a) what will the resulting pH be?
b) what would the pH be if no HEPES were present (that is, youmixed water and HCl)?
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a Given the total initial concentration of the buffer solution 01 M Since pH pKa salt acid 01 M 2 005 M Hence initial moles of HEPES salt MxV 005 molL x 0200 L 001
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