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You have a sample of ssDNA molecule that you will use as a primer for PCR....

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Biology

You have a sample of ssDNA molecule that you will use as aprimer for PCR. You dissolved the dried ssDNA peller in TE to atotal volume of 400 ul to make a \"stock DNA sample.\" You need todetermine the concentration (ug/ul) of this primer DNA in solution.To do this you took 4 ul from the \"stock DNA sample\" and addedthese to 996 ul of TE buffer in a cuvette, making a total of 1000ul in a cuvette. Next, you determined the absorbance of 260 nmlight for this 1 ml of diluted DNA in the cuvette and theA260 value is 0.014.

1) what is the concentration (ug/ul) of the DNAsolution in the cuvette?

2) what is the concentration (ug/ul) of theprimer DNA in the original Stock DNA sample?

3) what is the total amount (ug) of DNA in thestock DNA sample tube?

4) how many ul of the original \"stock DNAsample\" would you need to use in a PCR experiment that required atotal of 0.25 ug of DNA from this sample?

Answer & Explanation Solved by verified expert
3.6 Ratings (593 Votes)
Considering 1 OD 1 gL1 Concentration of the DNA solution in the cuvette    See Answer
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