Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. biology
  3. you have a viral stock of an unknown concentration

You have a viral stock of an unknown concentration and you want to determine the titer...

70.2K

Verified Solution

Question

Biology

You have a viral stock of an unknown concentration and you wantto determine the titer of the virus by plaque assay. Your lab matehas VERO cells growing in the lab and you decide to use them for aplaque assay. After infection with a range of dilutions, none ofthe plates showed plaques. Give 2 possible reasons why you did notget any plaques.

You repeat the plaque assay using CHO as the target cells thistime. You infect 3 wells with 0.1 ml of a 10-6 dilution of yourstock. The plates show 105, 95 and 100 plaques. What is the titerof your undiluted viral stock?

Answer & Explanation Solved by verified expert
4.4 Ratings (602 Votes)
During plaque assay of virus on VERO cells no plaques were formed Two possible reasons for nonoccurence of the plaques maybe 1 The virus may not be infecting the vero cells ie Vero    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Q

Write a Java program that uses the file EnglishWordList.txt which contains a collection of words in...

Programming
Q

Find the radius of the circle in the figure to the right EXXE The radius...

Basic Math
Q

Question 40 The quadratic function y f z has the graph shown 63 k 5...

Basic Math
Q

In this last step we are going to go back to our first reflection We...

Calculus
Q

Journakro entries for the following related transactions of Marville Heating & Air Company. Hofer to...

Accounting
Q

Baird Cameras, Inc. manufactures two models of cameras. Model ZM has a zoom lens; Model...

Accounting
Q

Which of the following is incorrect about the debt vs equity components of financial instruments?...

Accounting
Q

The Fair Value Adjustment account Answer A)...

Accounting
Q

It says the the preliminary cash balance and the additional loan is wrong in the...

Accounting
Q

Lone Star Company es una corporacin de ao calendario, y este ao Lone Star report...

Accounting
Q

Brief Exercise 9-13 x Your answer is incorrect. Try again. Bramble Inc. had beginning inventory...

Accounting
Q

9 Suppose that many stocks are traded in the market and that it is possible...

Finance