You have been provided a mixture containing four amino acids(Glu, His, Pro and Lys) to be separated by ion-exchangechromatography using a Dowex 50 cation-exchange, prepared in aloading buffer at pH 2.0. The following solutions are madeavailable:
The amino acid mixture in 0.04M citrate buffer at pH 2.0.
0.04M citrate buffer, pH 4.5
0.04M citrate buffer, pH 8.0
0.04M citrate buffer, pH 10.5
Is complete resolution of all amino acids achieved if thebuffers are allowed to elute through the column sequentially fromlowest to highest pH? Identify the order of elution and identifyany amino acids that may come off the column together if completeresolution is not achieved. Support your response with anexplanation.
Assuming the amino acids in the mixture will elute from the columnONLY at a solution pH that is above their respective pI.
