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You have just joined a lab and have obtained a flask with J558L cells. You take...

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Biology

You have just joined a lab and have obtained a flask with J558Lcells. You take an aliquot of the cells, dilute the aliquot withTrypan Blue (1:1), and count the cells using a hemocytometer andobtain the following numbers:

Transparent cells:Blue cells:
Quadrant 1521
Quadrant 2603
Quadrant 3582
Quadrant 4512
Quadrant 5571

1) What is the cell viability?

2) What is the density of the cells (living) in the culture?

Answer & Explanation Solved by verified expert
3.8 Ratings (725 Votes)
Answer Trypan blue dye when added to the cells a live cell will pump it out and become transparent Whereas dead cells will take the dye and stain blue Therefore all the transparent cells are live cells whereas blue cells are dead cells 1 Total    See Answer
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