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You have prepared a smear and stained it with crystal violet. Everything is fine until...

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Biology

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You have prepared a smear and stained it with crystal violet. Everything is fine until you examine your slide using the oil immersion lens and you find that you cannot focus on the cells because they are floating in the oil. You conclude that you made in mistake in which step? heat-fixing the slide cleaning the slide washing the excess stain off the slide spreading the bacteria onto the slide

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