You intend to use bacteria to produce ethanol for your spin-offbusiness in Palapye. The bacteria (strain: Ethanol Blue) you have,is a very poor ethanol producer and is very sensitive to ethanol.However, an industrial strain (strain: Ethanol Red), which has bothan elevated ethanol producing and a high ethanol tolerance trait,is available in your laboratory collections for use as a control.Both bacteria sporulate with relative ease. Note that an elevatedethanol producer is always resistant to elevated amounts ofethanol. a) Isn’t it strange that natural bacteria isolates produceethanol? Explain. [2 marks] b) Briefly describe 2 methods (takingadvantage of their sporulation ability only) you would use toimprove the fermentative capacity of Ethanol Blue. [4 marks] c)State any other methods other than evolutionary engineering andrecombinant DNA technology that can be used to develop the strain.[2 marks] d) Is there a correlation between ethanol production andtolerance? [2 marks] Total [10 marks]
