You plan on isolating a protein of interest by cutting a band out of a gel....

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Chemistry

You plan on isolating a protein of interest by cutting a bandout of a gel. Your protein is a phosphatase (a certain type ofenzyme) and you hope to measure its activity. Should you useSDS-PAGE or a native gel to isolate your protein? Why?

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Everything will work if you are able to visualize the band in the gel before recovery but the most important issue is what you want to do with the recovered protein If you are interested in trying to recover functional protein then you are far better off not using an SDS gel in the first See Answer

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You plan on isolating a protein of interest by cutting a band out of a gel....

50.1K

Verified Solution

Question

Chemistry

You plan on isolating a protein of interest by cutting a bandout of a gel. Your protein is a phosphatase (a certain type ofenzyme) and you hope to measure its activity. Should you useSDS-PAGE or a native gel to isolate your protein? Why?

Answer & Explanation Solved by verified expert

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