You purified the plasmid from an overnight cell culture and
dissolved the plasmid DNA in 100...
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Chemistry
You purified the plasmid from an overnight cell culture anddissolved the plasmid DNA in 100 μL 0.1M Tris buffer (pH8). Toquantify the plasmid, you took an absorbance measurement at 260 nmof a 1:50 dilution sample. The absorbance was 0.35. What is theconcentration of the undiluted plasmid in micrograms per mL?
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Ans The average extinction coefficient of a double stranded DNA is 0020 ug mL1cm1 that gives the value of 500 ug mL DNA concentration
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