You want to analyze the potential effects of Cisplatin,a well known chemotherapeutic agent on E-cadherin expression inASPC1 cells at different concentrations (1, 3 and 10 µM). (15pts)
a. Name and explain at least 3 techniques you can usefor this study in detail.
b. What will be your test conditions for thisstudy?
c. With the below information already given to you, howmany microliters of the drug will you add to each condition if youare using a 10 cm dish for cell culture?
- Main stock of Cisplatin is 10 mM
- Test concentrations will be 1, 3 and 10µM.
