Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. chemistry
  3. you work in a medical research lab with a biochemi

You work in a medical research lab with a biochemist who asks you to make a...

60.2K

Verified Solution

Question

Chemistry

You work in a medical research lab with a biochemist who asksyou to make a 1.00 L phosphate buffer stock solution with pH = 7.4.The total phosphorus concentration is to be 0.250 M. In thestoreroom, there are the following reagents: (1) a 3.00 Mphosphoric acid stock solution, (2) solid sodium dihydrogenphosphate, (3) solid sodium monohydrogen phosphate, (4) solid,sodium phosphate, (5) a 3.00 M HCl(aq) stock solution, (6) a 3.00 MNaOH(aq) stock solution, and (7) deionized water.

(i) (2 pts) To make an optimum buffer, it is important that thepKa of the acid part is close to the target pH of the buffer. Whichphosphate species has a pKa value closest to pH = 7.4?

(ii) (4 pts) Choose this phosphate species (in i) as the onlyphosphorus component. What amounts of this substance and any otherreagents (grams of solid(s); mL of any solution(s) and water) wouldyou need to make the buffer with pH = 7.4?

(iii)(4 pts) Choose this phosphate species (in i) and either itsconjugate acid or base as the only two phosphorus components. Whatamounts of this substance, its conjugate acid or base, and water(grams of solid(s); mL of any solution(s) and water) would you needto make the buffer with pH = 7.4?

Answer & Explanation Solved by verified expert
4.1 Ratings (556 Votes)
Using HendersenHasselbalck equation pH pKa logbaseacid pKa2 for H3PO4 72 74 72 logbaseacid base 1585acid acid base 025 base 025 acid 025 acid 1585acid acid 0097 M    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Zin AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Q

2) Describe a health concern you have, that you feel comfortable sharing with the class (example:...

Psychology
Q

Target due 4 12 23 37 What was the first concession that the United States...

Biology
Q

7 AABC is an obtuse triangle with obtuse angle A ADEF is an acute triangle...

Geometry
Q

manager of the customer service department of an e-commerce company wants to know the average...

Basic Math
Q

You pick a card at random put it back and then pick another card at...

Calculus
Q

2 3x 12x 3x 12

Algebra
Q

Dobson Manufacturing Company uses a job order cost system with manufacturing overhead...

Accounting
Q

answer all three questions please Match the type of...

Accounting
Q

Q2: mprig a firm fiil iditr with its own past performance or even with the...

Accounting
Q

On January 1,2025, Marigold Company purchased 12% bonds having a maturity value of $278,000 for...

Accounting
Q

Liquidation schedule-positive capital accounts The ABC partnership reports the following condensed balance sheet: ...

Accounting
Q

When the "kiddie tax" is imposed, what is the tax treatment of the resulting income?...

Accounting