Your lab is studying gene expression of the tryptophan operon inthe bacterium E. coli. In order to facilitate this study, you havecloned the Trp-operon, excised the five coding genes (TrpA, B, C,D, and E), and replaced them with a copy of the reporter gene GFP(green fluorescent protein). The resulting recombinant DNA wasinserted into an F' plasmid, and used to transform a number ofdifferent strains of E. coli. For each of the scenarios listedbelow, predict what the bacterial colonies would look like, andexplain why you would obtain these results.
a. A \"wild type\" strain of E. coli, transformed by the F'Trp/GFP reporter plasmid, is plated out on a selective mediumlacking tryptophan.
b. A \"wild type\" strain of E. coli, transformed by the F'Trp/GFP reporter plasmid, is plated out on tryptic soy medium.
c. A trpR mutant strain of E. coli, transformed by the F'Trp/GFP reporter plasmid, is plated out on tryptic soy medium.
