You’ve identified an antigenic protein unique to multidrugresistant Staphylococcus aureus (MRSA). You’d like tocreate a MRSA vaccine based on an in vitro transcribedmRNA. Several answers will be acceptable, sincethere are several technical approaches to carry out the same tasks.Specifically:
(1 pt) What molecular method could you use toclone the gene from S. aureus? (Name a specific approachfrom earlier in the course).
(1 pt) What will be the components of your IVTmRNA itself?
(1 pt) What steps could you take to reduce theimmunogenicity of the IVT mRNA?
(1 pt) What is one way you might deliver theIVT mRNA into a patient?
(1 pt) You find that the S. aureusprotein is poorly expressed in human cells. How could you changeyour
        mRNA sequenceto boost ex
