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  3. 4. you want to clone a 1.5 kb cdna. which vectors

4. You want to clone a 1.5 kb cDNA. Which vectors discussed would be appropriate to...

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Biology

4. You want to clone a 1.5 kb cDNA. Which vectors discussedwould be appropriate to use? Which would be

inappropriate? Explain your answer.

5. You want to make a genomic library with DNA fragmentsaveraging about 85 kb in length. Which vectors discussed

would be appropriate to use? Which would be inappropriate?Explain your answer.

6. Describe the process of cloning a DNA fragment into themultiple cloning site of the vector pUC18. How would you

screen for clones that contain an insert? Explain the underlyingprinciple between blue-white screening.

Answer & Explanation Solved by verified expert
4.0 Ratings (459 Votes)
The cloning vectors which are discussed Options are not mentioned in the question however I will answer the question based on the general cloning vectors and their sizes a Plasmid vector 01 to 10 kb insert b Bacterial artificial chromosome insert size 35 300 kb c Yeast artificial chromosome insert size 100 1000kb d bacteriophage 4 5 kb e cosmid insert size 30 50 kb 4 The insert size is 15 kb So the plasmid vector is most suitable for such cloning Oher vectors have    See Answer
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