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A random multiple choice question abiut the topic, Dideoxy DNA Sequencing of Sanger?

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Biology

Arandom multiple choice question abiut the topic, Dideoxy DNASequencing of Sanger?

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Why dont we see the template strand on the gelThe denaturing step and the urea in the gel destroyed itThe template strand lacks radioactivelyfluorescentlylabeled ddNTPThe template strand is too large to appear as a band on thegelAll of the following are disadvantages of the Sanger methodexceptIt requires PCR amplification prior to sequencingIt is limited by the resolution of polyacrylamide gelelectrophoresisIt is expensiveIt has low accuracy compared to most othermethodsWhy must DNA be    See Answer
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