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In automated DNA sequencing reaction by the Sanger method, which of the following is NOT required? fluorescently-labeled...

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Biology

In automated DNA sequencing reaction by the Sanger method, whichof the following is NOT required?

fluorescently-labeled dideoxynucleotides

deoxynucleotides

DNA polymerase

a DNA primer

none of the above (i.e. all the above are required in thereaction)

Answer & Explanation Solved by verified expert
3.9 Ratings (697 Votes)
None of the above DNA sequencing is the process for the determination of the order of nucleotides in DNA Sanger Sequencing or dideoxy sequencing is the most common method used in DNA sequencing which is developed by Frederick Sanger in 1977 This is sequencing by synthesis method In this process the DNA to be sequenced is mixed with a radiolabeled primer    See Answer
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