1. Based on the pI of Taq, what pH buffer would you have to useto get Taq polymerase to stick to an anion exchanger?
Briefly discuss the advantages and disadvantages of using a saltor pH in eluting proteins from an ion-exchange column.
Please place details explaining how you arrived to ech answerfor question 1
Answer & Explanation
Solved by verified expert
4.2 Ratings (640 Votes)
Given Taq protein Molecular weight 9391016 Theoretical pI 603 When the pH is lower than the pI the amino acid will carry a net positive charge When the pH is higher than pI then the amino acid carries a net negative charge We need to use anion exchanger therefore molecule must be
See Answer
Get Answers to Unlimited Questions
Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!
Membership Benefits:
Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Zin AI - Your personal assistant for all your inquiries!
