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Please explain why B is the correct answer, I know on the 5'---->3' it goes reverse and...

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Biology

Please explain why B is the correct answer, I know on the5'---->3' it goes reverse

and 3'-->5' it goes forward.......I got lost after this.Please explain. Will give a thumbs up.

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Which of the following sets of primers could you use to amplifythe target DNA sequence below, which is part of the lastprotein-coding exon of the gene involved in cystic fibrosis?

5’- ggctaagatctgaattttccgag … ttgggcaataatgtagcgcctt - 3’

3’- ccgattctagacttaaaaggctc … aacccgttattacatcgcggaa – 5’

a) 5’ GGAAAATTCAGATCTTAG 3’;

5’ TGGGCAATAATGTAGCGC 3’

b) 5’ GCTAAGATCTGAATTTTC 3’;

3’ ACCCGTTATTACATCGCG 5’

c) 3’ GATTCTAGACTTAAAAGGC 5’;

3’ ACCCGTTATTACATCGCG 5’

d) 5’ GCTAAGATCTGAATTTTC 3’;

5’ TGGGCAATAATGTAGCGC 3’.

Answer & Explanation Solved by verified expert
3.8 Ratings (641 Votes)
Firstly want to explain that the DNA template is read in 3 to 5 direction and a new strand is synthesized in the 5 to 3 direction RNA polymerase uses template strand DNA strands to make a complementary RNA    See Answer
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